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KMID : 1024420140180040406
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Food Engineering Progress
2014 Volume.18 No. 4 p.406 ~ p.412
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A Study on Isolation of Polysaccharides from Angelica gigas Nakai by Enzyme Treatments
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Lee Hee-Jung
Jeong Heon-Sang
Park Chun-Geon
Lee Jeong-Hoon
Park Chun-Geon
Kim Chong-Tai
Choi Ae-Jin
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Abstract
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This study conducted enzyme treatments to maximize the extraction of polysaccharide components in Angelica gigasNakai (A. gigas). An extraction method of polysaccharides was established by using different enzyme treatments forthe degradation of plant cell walls. Total carbohydrates and removed starch of A. gigas increased after enzyme treat-ments such as Viscozyme (V), Alcalase (A), Flavorzyme (F) and Termamyl 120L (T). The yield and total carbo-hydrates of VAFT were 12.20% and 76.80%, respectively; an increase of 1.4 times that of T and AFT and showeda significant difference (p<0.05). Removed starch content also increased by more than three times the control inwater-extractable polysaccharides. Non-starch polysaccharide content of VAFT was 22.58%; it increased about 1.7times that of the control (12.84%) and it was the highest compared to T and AFT. Arabinose and galactose, whichwere the main components of A. gigas polysaccharides, increased from 1.5 to 2.2 times the control in the enzymetreatment groups. The high molecular weight fraction I of the control, depending on enzyme treatment conditions,was about 491,282 Da. It degraded into a low molecular weight fraction ranged from 282,459 to 16,362 Da. Themolecular weight of polysaccharides treated by the VAFT decreased to an average of 13,000 Da. So, VAFT wasselected as the optimizing condition for extracting water extractable polysaccharides from A. gigas. As a result ofthis study, it is expected that A. gigas will be widely used for industrial materials and useful to develop and researchmaterials of different medicinal plants which are not yet known.
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KEYWORD
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Angelica gigas Nakai, enzyme treatment, medicinal plants, polysaccharides
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